Rare Allele 29 at Locus D2S1338 Observed During Routine Casework in Bulgarian Population

In this work, we report a rare allele 29 at locus D2S1338, established during routine forensic practice in case of first-degree kinship (parenthood). This variant to the best our knowledge, is reported for first time Bulgarian population. So far it has not been registered studies frequencies same 20 population groups Europe and Asia. The presentation similar genotyping findings relating rare/unexpected genetic variation very important examination documentation such anomalies. analysis performed 16 STR loci: SE33, D16S539, D18S51, TH01, D12S391, D3S1358, FGA, vWA, D21S11, D1S1656, D2S441, D8S1179, D19S433, D22S1045, D10S1248 2 sex determination systems – Amelogenin Y indel, set NGM DetectTM PCR Amplification Kit (Applied Biosystems). use allelic witnesses diagnostic mandatory standard fragment DNA analysis. witness contains well-known preset alleles examined locus. Establishing that are outside factory importance broadening scope heightening accuracy Rare variants significantly increase strength discrimination when profiles compared. regard, any new information about emergence detected particular group.